FIGURE

Fig. 5

ID

ZDB-FIG-151106-4

Publication

Boije et al., 2015 - The Independent Probabilistic Firing of Transcription Factors: A Paradigm for Clonal Variability in the Zebrafish Retina

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

HCs Are Generated as the Intersection between the Lhx1 and Ptf1a Lineages

(A) The retina of a quadruple transgenic zebrafish (Atoh7-gapGFP; Ptf1a-dsRed; Lhx1-GFP; Crx-gapCFP) at 96 hpf. Individual channels with arrowheads illustrating the overlap between GFP and dsRed.

(B) Quadruple transgenic retina at 48 hpf. Arrows indicate Lhx1-GFP, Ptf1a-dsRed double positive cells, i.e., HCs. Individual channels reveal overlap between Crx-CFP and Lhx1-GFP, as indicated by arrowheads. pPR, putative PR.

(C) Combinatorial expression in the quadruple transgenic allows identification of the different cell types in a dissociated sample. Channel designation below is valid for both (C) and (D).

(D) Lhx1-positive PR cell.

(E) Percentages of cells that express Lhx1, Ptf1a, or both Lhx1 and Ptf1a.

The scale bar in (A) represents 20 µm and is also valid for (B), while the scale bar in (C) represents 5 µm and is also valid for (D).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 34(5), Boije, H., Rulands, S., Dudczig, S., Simons, B.D., Harris, W.A., The Independent Probabilistic Firing of Transcription Factors: A Paradigm for Clonal Variability in the Zebrafish Retina, 532-43, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell