PUBLICATION

The Independent Probabilistic Firing of Transcription Factors: A Paradigm for Clonal Variability in the Zebrafish Retina

Authors
Boije, H., Rulands, S., Dudczig, S., Simons, B.D., Harris, W.A.
ID
ZDB-PUB-150908-7
Date
2015
Source
Developmental Cell   34(5): 532-43 (Journal)
Registered Authors
Dudczig, Stefanie, Harris, William A.
Keywords
none
MeSH Terms
  • Animals
  • Cell Differentiation/physiology
  • Cell Lineage/physiology
  • Gene Expression Regulation, Developmental/physiology*
  • Retina/cytology*
  • Retina/metabolism*
  • Stem Cells/cytology*
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed
26343455 Full text @ Dev. Cell
Abstract
Early retinal progenitor cells (RPCs) in vertebrates produce lineages that vary greatly both in terms of cell number and fate composition, yet how this variability is achieved remains unknown. One possibility is that these RPCs are individually distinct and that each gives rise to a unique lineage. Another is that stochastic mechanisms play upon the determinative machinery of equipotent early RPCs to drive clonal variability. Here we show that a simple model, based on the independent firing of key fate-influencing transcription factors, can quantitatively account for the intrinsic clonal variance in the zebrafish retina and predict the distributions of neuronal cell types in clones where one or more of these fates are made unavailable.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping