FIGURE

Fig. 5

ID

ZDB-FIG-151030-16

Publication

Miesfeld et al., 2015 - Yap and Taz regulate retinal pigment epithelial cell fate

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Fig. 5

Tead-binding-deficient yap^?TB/?TB mutants lack RPE but maintain yap mRNA and Yap protein levels. (A) The Tead-binding-deficient yap^?TB/?TB zebrafish mutant. (B-D) 48hpf whole eyes showing that yap^-/- and yap^?TB/?TB mutants lose a subset of RPE cells. Dashed lines indicate the border of the eye. (E) qRT-PCR analysis of whole embryos at 32hpf revealing a decrease in yap (1.6-fold, *P=0.0052) and taz (1.15-fold, *P=0.0038) mRNA in yap^?TB/?TB mutants. Dotted line indicates the normalized expression levels of yap and taz in wild-type embryos. An unpaired t-test was performed and statistical significance was determined using the Holm-Sidak method. Error bars indicate s.e.m. (F-G′′′) Yap protein expression in yap^+/?TB (F-F′′′) and yap^?TB/?TB (G-G′′′) at 28hpf. Yap protein is present in flattened RPE nuclei (arrows) and periocular cells in yap^+/?TB and yap^?TB/?TB embryos.

Expression Data

Antibody:	Ab1-yap1
Fish:	yap1^mw69/mw69 yap1^mw69/+
Anatomical Terms:	periocular mesenchyme presumptive retinal pigmented epithelium nucleus
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	yap1^mw48/mw48 yap1^mw69/mw69
Observed In:	retinal pigment epithelium development retinal pigmented epithelium
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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