FIGURE

Fig. 3

ID

ZDB-FIG-151030-14

Publication

Miesfeld et al., 2015 - Yap and Taz regulate retinal pigment epithelial cell fate

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Fig. 3

yap mRNA and Yap protein levels are decreased and Taz protein increased in yap^-/- embryos. (A-B′′′) Yap immunoreactivity in wild-type and yap^-/- eyes at 28hpf. Yap protein is present in flattened RPE nuclei (arrows) and periocular mesenchyme (POM) in yap^+/- embryos, whereas nuclear Yap staining is absent in the yap^-/- mutant. (C) qRT-PCR analysis of whole embryos at 32hpf showing a decrease in yap (3-fold, *P=0.0002) and taz (1.5-fold, *P=0.0270) mRNA in yap^-/- mutants. Dotted line indicates normalized expression levels of yap and taz in wild-type embryos. An unpaired t-test was performed and statistical significance determined using the Holm-Sidak method. Error bars represent s.e.m. (D) Western blot showing Taz protein (~52 kDa) in wild-type and its absence in taz^-/- adult heart tissue. (E-G′′′) Taz immunoreactivity in wild-type, taz^-/- and yap^-/- embryos at 28hpf. (H) Western blot of Taz protein from 2dpf wild-type (n=20) and yap^-/- mutant (n=20) whole embryos.

Expression Data

Antibodies:	Ab1-wwtr1 Ab1-yap1
Fish:	WT wwtr1^mw49/mw49 yap1^mw48/mw48 yap1^mw48/+
Anatomical Terms:	heart periocular mesenchyme presumptive retinal pigmented epithelium presumptive retinal pigmented epithelium nucleus whole organism
Stage Range:	Prim-5 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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