ZFIN Image: Miesfeld et al., 2015, Fig. 5

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Figure Caption

Fig. 5

Tead-binding-deficient yap^?TB/?TB mutants lack RPE but maintain yap mRNA and Yap protein levels. (A) The Tead-binding-deficient yap^?TB/?TB zebrafish mutant. (B-D) 48hpf whole eyes showing that yap^-/- and yap^?TB/?TB mutants lose a subset of RPE cells. Dashed lines indicate the border of the eye. (E) qRT-PCR analysis of whole embryos at 32hpf revealing a decrease in yap (1.6-fold, *P=0.0052) and taz (1.15-fold, *P=0.0038) mRNA in yap^?TB/?TB mutants. Dotted line indicates the normalized expression levels of yap and taz in wild-type embryos. An unpaired t-test was performed and statistical significance was determined using the Holm-Sidak method. Error bars indicate s.e.m. (F-G′′′) Yap protein expression in yap^+/?TB (F-F′′′) and yap^?TB/?TB (G-G′′′) at 28hpf. Yap protein is present in flattened RPE nuclei (arrows) and periocular cells in yap^+/?TB and yap^?TB/?TB embryos.

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Acknowledgments

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