ZFIN ID: ZDB-FIG-151016-28
Jin et al., 2014 - Prostaglandin signalling regulates ciliogenesis by modulating intraflagellar transport. Nature cell biology   16(9):841-51 Full text @ Nat. Cell Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to Day 4
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to Day 4

Fig. 5 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 WT + MO1-ptgs1 standard conditions Prim-5 heart tube ISH
WT + MO2-ptger4a standard conditions Prim-5 heart tube ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT control 5-9 somites Kupffer's vesicle motile cilium
IHC Day 4 lateral crista kinocilium
IHC WT + MO1-ptgs1 standard conditions 5-9 somites Kupffer's vesicle motile cilium
IHC Day 4 lateral crista kinocilium
IHC WT + MO1-ptgs1 chemical treatment: prostaglandin E2 5-9 somites Kupffer's vesicle motile cilium
IHC WT + MO1-ptgs1 + MO2-ptgs2a chemical treatment: prostaglandin E2 Day 4 lateral crista kinocilium
IHC WT + MO2-ptger4a standard conditions 5-9 somites Kupffer's vesicle motile cilium
IHC Day 4 lateral crista kinocilium
IHC WT + MO2-ptger4a chemical treatment: forskolin 5-9 somites Kupffer's vesicle motile cilium
IHC Day 4 lateral crista kinocilium
IHC WT + MO2-ptger4a chemical treatment: prostaglandin E2 5-9 somites Kupffer's vesicle motile cilium
IHC WT + MO2-ptgs2a standard conditions Day 4 lateral crista kinocilium
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ptgs1 standard conditions 5-9 somites cilium assembly decreased process quality, abnormal
5-9 somites Kupffer's vesicle has fewer parts of type Kupffer's vesicle motile cilium, abnormal
5-9 somites Kupffer's vesicle motile cilium decreased length, abnormal
Prim-5 heart bilateral symmetry, abnormal
Prim-5 heart looping process quality, abnormal
Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth brain ventricular system edematous, abnormal
Protruding-mouth otic vesicle has extra parts of type otolith, abnormal
Protruding-mouth post-vent region curved ventral, abnormal
Protruding-mouth trunk curved ventral, abnormal
Day 4 cilium assembly decreased process quality, abnormal
Day 4 lateral crista has fewer parts of type lateral crista kinocilium, abnormal
WT + MO1-ptgs1 chemical treatment: prostaglandin E2 5-9 somites cilium assembly decreased process quality, abnormal
5-9 somites Kupffer's vesicle has fewer parts of type Kupffer's vesicle motile cilium, abnormal
5-9 somites Kupffer's vesicle motile cilium length, normal
Prim-5 heart bilateral symmetry, normal
Prim-5 heart looping process quality, normal
WT + MO1-ptgs1 + MO2-ptgs2a standard conditions Protruding-mouth brain ventricular system edematous, abnormal
WT + MO1-ptgs1 + MO2-ptgs2a chemical treatment: prostaglandin E2 Protruding-mouth brain ventricular system morphology, normal
Day 4 lateral crista has normal numbers of parts of type lateral crista kinocilium, normal
WT + MO2-ptger4a standard conditions 5-9 somites cilium assembly decreased process quality, abnormal
5-9 somites Kupffer's vesicle has fewer parts of type Kupffer's vesicle motile cilium, abnormal
5-9 somites Kupffer's vesicle motile cilium decreased length, abnormal
Prim-5 heart bilateral symmetry, abnormal
Prim-5 heart looping process quality, abnormal
Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth brain ventricular system edematous, abnormal
Protruding-mouth otic vesicle has extra parts of type otolith, abnormal
Protruding-mouth post-vent region curved ventral, abnormal
Protruding-mouth trunk curved ventral, abnormal
Day 4 cilium assembly decreased process quality, abnormal
Day 4 lateral crista has fewer parts of type lateral crista kinocilium, abnormal
WT + MO2-ptger4a chemical treatment: forskolin 5-9 somites Kupffer's vesicle has fewer parts of type Kupffer's vesicle motile cilium, abnormal
5-9 somites Kupffer's vesicle motile cilium decreased length, abnormal
Prim-5 heart bilateral symmetry, normal
Prim-5 heart looping process quality, normal
Protruding-mouth brain ventricular system morphology, normal
Day 4 lateral crista has normal numbers of parts of type lateral crista kinocilium, normal
WT + MO2-ptger4a chemical treatment: prostaglandin E2 5-9 somites cilium assembly decreased process quality, abnormal
5-9 somites Kupffer's vesicle has fewer parts of type Kupffer's vesicle motile cilium, abnormal
5-9 somites Kupffer's vesicle motile cilium decreased length, abnormal
Prim-5 heart bilateral symmetry, abnormal
Prim-5 heart looping process quality, abnormal
Protruding-mouth brain ventricular system edematous, abnormal
WT + MO2-ptgs2a standard conditions 5-9 somites Kupffer's vesicle has normal numbers of parts of type Kupffer's vesicle motile cilium, normal
5-9 somites Kupffer's vesicle motile cilium length, normal
Prim-5 heart bilateral symmetry, normal
Prim-5 heart looping process quality, normal
Protruding-mouth brain ventricular system edematous, abnormal
Day 4 cilium assembly decreased process quality, abnormal
Day 4 lateral crista has fewer parts of type lateral crista kinocilium, abnormal
Full text @ Nat. Cell Biol.