Fig. 3
- ID
- ZDB-FIG-150910-3
- Publication
- Fortuna et al., 2015 - Vascular Mural Cells Promote Noradrenergic Differentiation of Embryonic Sympathetic Neurons
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Absence of Blood Flow Perturbs Vascular Maturation and SN Differentiation (A, B, D, and E) Confocal images of control (A and D) or tnnt2 morpholino-injected (B and E) Tg(kdrl:EGFP)la116 zebrafish embryos immunostained for GFP (green), HU (red), and TH (blue). Dorsal views (anterior is to the left). HU+/TH+ SNs are pseudocolored in white and indicated (arrows). Tnnt2 morphants display defective remodeling of the LDA-DA and misalignment of HU+/TH+ neurons. (C and F) Quantification of SNs (HU+/TH+) in control and tnnt2 morpholino-injected embryos at 48 (C) and 60 hpf (F). Data were calculated from three independent experiments. NS, not significant; error bars indicate SD. Hpf, hours post-fertilization. Scale bar, 50 µm (A, B, D, and E). |