ZFIN Image: Fortuna et al., 2015, Fig. 3

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Figure Caption

Fig. 3

Absence of Blood Flow Perturbs Vascular Maturation and SN Differentiation

(A, B, D, and E) Confocal images of control (A and D) or tnnt2 morpholino-injected (B and E) Tg(kdrl:EGFP)^la116 zebrafish embryos immunostained for GFP (green), HU (red), and TH (blue). Dorsal views (anterior is to the left). HU⁺/TH⁺ SNs are pseudocolored in white and indicated (arrows). Tnnt2 morphants display defective remodeling of the LDA-DA and misalignment of HU⁺/TH⁺ neurons.

(C and F) Quantification of SNs (HU⁺/TH⁺) in control and tnnt2 morpholino-injected embryos at 48 (C) and 60 hpf (F). Data were calculated from three independent experiments.

NS, not significant; error bars indicate SD. Hpf, hours post-fertilization. Scale bar, 50 µm (A, B, D, and E).

Acknowledgments

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