FIGURE

Fig. 2

ID

ZDB-FIG-150716-15

Publication

Xiao et al., 2015 - High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish

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Fig. 2

The phenotype of homozygous Tg[gSAGFF202A] fish. (A) Heterozygous Tg[gSAGFF202A;UAS:EGFP;SILL:mCherry] 5-dpf larva treated with 4-Di-2-ASP, revealing the normal pattern of neuromasts in its posterior lateral line (five neuromasts within the dashed rectangle). (B) A homozygous Tg[gSAGFF202A;UAS:EGFP;SILL:mCherry] fish at 5dpf possesses around twice as many neuromasts (ten neuromasts within the dashed rectangle). (C,E) The normal expression pattern of the posterior ganglion and lateral line in heterozygous Tg[gSAGFF202A;UAS:EGFP;SILL:mCherry] fish at 5dpf. (D,F) At 5dpf, the abnormal expression pattern of the posterior ganglion and lateral line in homozygous Tg[gSAGFF202A;UAS:EGFP;SILL:mCherry] fish, in which Schwann cells cannot migrate far away from the posterior ganglion. (G) Lattice light-sheet imaging reveals details of the axonal defasciculation in homozygous Tg[gSAGFF202A;UAS:EGFP;SILL:mCherry] larvae at 5dpf. (H) BLAST (basic local alignment search tool) shows the insertion of Gal4FF within the ErbB2 gene. (I) The integration site indicated by red nucleotides. Scale bars: 150µm (A,B), 50µm (C,D) and 10µm (E-G).

Expression Data

Genes:	EGFP mCherry
Fish:	erbb2^{nksagff202aTg/+}; bc3Tg; nkuasgfp1aTg erbb2^{nksagff202aTg/nksagff202aTg}; bc3Tg; nkuasgfp1aTg
Anatomical Terms:	lateral line myelinating Schwann cell posterior lateral line afferent neuron posterior lateral line myelinating Schwann cell posterior lateral line ganglion afferent neuron posterior lateral line ganglion myelinating Schwann cell
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	erbb2^{nksagff202aTg/nksagff202aTg}; bc3Tg; nkuasgfp1aTg
Observed In:	afferent neuron afferent neuron axon myelinating Schwann cell neuromast posterior lateral line myelinating Schwann cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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