ZFIN ID: ZDB-FIG-150716-20
Xiao et al., 2015 - High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish. Disease models & mechanisms   8:553-564 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Day 5 to Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 7

Regenerating axons followed Schwann cell processes. (A) Frames captured from time-lapse supplementary material Movie 4 of a Tg[gSAGFF202A;UAS:EGFP;SILL:mCherry] larva with severed axons, leaving a glial gap. Time elapsed from the first frame of the movie are, from left to right and top to bottom: 00:04, 00:20, 00:30, 00:40, 00:45, 00:46, 00:49, 00:52. Pictures were taken every 8min, starting 4h after axon severing. At 16hpt (00:45 time point), axons started to regrow when Schwann cells filled the gap. (B) An example of partially defasciculated axons at the site of injury. (C) A quadruple transgenic Tg[gSAGFF202A;UAS:EGFP;SILL:mCherry;Brn3c:mEGFP] larva starting at 6dpf and following 0.5hpt, 4hpt, 24hpt, 48hpt and 72hpt after axon severing and ablation of lateral Schwann cells. At 24hpt, regenerating axons re-innervated the neuromast with lateral Schwann cells, but failed to re-innervate neuromasts devoid of lateral Schwann cells even at 72hpt. Asterisks mark pigment cells. Arrows point to ablated lateral Schwann cells. Dash frame indicates the site of the cut. Scale bars: 25┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg transection: axon: neuron Day 5 posterior lateral line myelinating Schwann cell IFL
Day 5 posterior lateral line myelinating Schwann cell IHC
erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg; s356tTg control Day 5 posterior lateral line myelinating Schwann cell IFL
erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg; s356tTg transection: lateral line nerve, ablation: myelinating Schwann cell Day 5 to Days 7-13 posterior lateral line myelinating Schwann cell IFL
GFP erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg; s356tTg control Day 5 neuromast hair cell IFL
Day 5 posterior lateral line neuromast IFL
erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg; s356tTg transection: lateral line nerve, ablation: myelinating Schwann cell Day 5 to Days 7-13 neuromast hair cell IFL
Day 5 to Days 7-13 posterior lateral line neuromast IFL
mCherry erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg transection: axon: neuron Day 5 posterior lateral line afferent neuron IHC
Day 5 posterior lateral line afferent neuron IFL
erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg; s356tTg control Day 5 posterior lateral line afferent neuron IFL
erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg; s356tTg transection: lateral line nerve, ablation: myelinating Schwann cell Day 5 to Days 7-13 posterior lateral line afferent neuron IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-cldnk IHC erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg transection: axon: neuron Day 5 posterior lateral line myelinating Schwann cell
Phenotype Details
Fish Conditions Stage Phenotype
erbb2nksagff202aTg; bc3Tg; nkuasgfp1aTg; s356tTg transection: lateral line nerve, ablation: myelinating Schwann cell Day 5 - Days 7-13 posterior lateral line axon regeneration decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.