FIGURE

Fig. 7

ID

ZDB-FIG-150716-20

Publication

Xiao et al., 2015 - High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish

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Fig. 7

Regenerating axons followed Schwann cell processes. (A) Frames captured from time-lapse supplementary material Movie 4 of a Tg[gSAGFF202A;UAS:EGFP;SILL:mCherry] larva with severed axons, leaving a glial gap. Time elapsed from the first frame of the movie are, from left to right and top to bottom: 00:04, 00:20, 00:30, 00:40, 00:45, 00:46, 00:49, 00:52. Pictures were taken every 8min, starting 4h after axon severing. At 16hpt (00:45 time point), axons started to regrow when Schwann cells filled the gap. (B) An example of partially defasciculated axons at the site of injury. (C) A quadruple transgenic Tg[gSAGFF202A;UAS:EGFP;SILL:mCherry;Brn3c:mEGFP] larva starting at 6dpf and following 0.5hpt, 4hpt, 24hpt, 48hpt and 72hpt after axon severing and ablation of lateral Schwann cells. At 24hpt, regenerating axons re-innervated the neuromast with lateral Schwann cells, but failed to re-innervate neuromasts devoid of lateral Schwann cells even at 72hpt. Asterisks mark pigment cells. Arrows point to ablated lateral Schwann cells. Dash frame indicates the site of the cut. Scale bars: 25µm.

Expression Data

Genes:	EGFP GFP mCherry
Antibody:	Ab2-cldnk
Fish:	erbb2^{nksagff202aTg}; bc3Tg; nkuasgfp1aTg erbb2^{nksagff202aTg}; bc3Tg; nkuasgfp1aTg; s356tTg
Conditions:	transection: axon: neuron ablation: myelinating Schwann cell, transection: lateral line nerve
Anatomical Terms:	neuromast hair cell posterior lateral line afferent neuron posterior lateral line myelinating Schwann cell posterior lateral line neuromast
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	erbb2^{nksagff202aTg}; bc3Tg; nkuasgfp1aTg; s356tTg
Condition:	ablation: myelinating Schwann cell, transection: lateral line nerve
Observed In:	posterior lateral line axon regeneration
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

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