Whole Mount in situ Hybridization analysis of goosecoid and chordin expression in probiotic treated and control embryos. Analysis of goosecoid expression in embryos treated with the probiotic L. rhamnosus compared to control embryos at 4, 8, 12 and 48 hpf and of chordin expression in the same groups at 4, 8 and 12hpf. According to q-PCR results both markers are up-regulated at 4 hpf in probiotic treated embryos compared to controls, although the over-expression is more evident for chordin. At 8 hpf chordin expression seems to be reduced ventrally in probiotic treated group compared with controls. No clear differences were detected at the other developmental stages (scale bar: 200 µm).
|