PUBLICATION

Beneficial Bacteria Affect Danio rerio Development by the Modulation of Maternal Factors Involved in Autophagic, Apoptotic and Dorsalizing Processes

Authors
Miccoli, A., Gioacchini, G., Maradonna, F., Benato, F., Skobo, T., Carnevali, O.
ID
ZDB-PUB-150403-3
Date
2015
Source
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology   35: 1706-1718 (Journal)
Registered Authors
Benato, Francesca, Carnevali, Oliana, Skobo, Tatjana
Keywords
none
MeSH Terms
  • bcl-2-Associated X Protein/metabolism
  • Female
  • Lacticaseibacillus rhamnosus/physiology*
  • Animals
  • Apoptosis Regulatory Proteins/metabolism
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/microbiology
  • Apoptosis*
  • Probiotics/pharmacology
  • Embryonic Development/drug effects
  • Zebrafish/growth & development
  • Zebrafish/microbiology*
  • Autophagy*
  • Caspase 3/metabolism
  • Microtubule-Associated Proteins/metabolism
  • Adaptor Proteins, Signal Transducing/metabolism
  • Proto-Oncogene Proteins c-bcl-2/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Gene Expression Regulation
  • Male
  • RNA, Messenger/metabolism
(all 23)
PubMed
25833742 Full text @ Cell Physiol. Biochem.
Abstract

Background: Probiotic strains have been recognized to exert important roles in many biological systems, including immune response, growth, development and reproduction. However, to date, no studies have focused either on the relation among probiotics and maternal factors or on probiotics' ability to qualitatively and/or quantitatively modulate maternal transcripts.

Methods In this study, the effects of Lactobacillusrhamnosus administered to parental fish on the control of maternal factors involved in autophagic, apoptotic and dorsalizing processes during zebrafish embryo development were assessed through q-PCRs, WMISH and TUNEL assay.
Results The results we obtained show that probiotic induced significant changes in both maternal and zygotic mRNA levels involved in embryo development. The maternal autophagy-regulating genes herein investigated -ambra1a, ambra1b, beclin, lc3-, as well as those involved in the apoptotic process -caspase3, bcl2, bax- were modulated in disfavor and favor of the treated group, respectively. Also, the key transcripts ruling the dorsalizing process -goosecoid and chordin- were subject to a significant regulation of their gene expression.
Conclusion The results we acquired demonstrated that parentally administered Lactobacillusrhamnosus is able to modulate important physiological processes involved in zebrafish embryo development.

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Citation
Miccoli, A., Gioacchini, G., Maradonna, F., Benato, F., Skobo, T., Carnevali, O. (2015) Beneficial Bacteria Affect Danio rerio Development by the Modulation of Maternal Factors Involved in Autophagic, Apoptotic and Dorsalizing Processes. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 35:1706-1718.