ZFIN ID: ZDB-FIG-150526-4
Ye et al., 2015 - glucagon is essential for alpha cell transdifferentiation and beta cell neogenesis. Development (Cambridge, England)   142:1407-17 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Day 4 to Days 7-13

Fig. 4

glucagon is required for islet development. (A) Endodermal organs isolated from 4-dpf larvae. p, pancreas; li, liver; ib, intestinal bulb. (B) qPCR of insulin (ins) and glucagon (gcga) in control and ablated larvae. ins expression was diminished and gcga expression transiently increased during regeneration (n=3 independent experiments). (C-E) gcgaMO blocks glucagon protein expression and increases the α to β cell ratio. (C-D′′) Confocal projections of 5-dpf control MO-injected (C) and gcgaMO-injected (D) Tg(gcga:GFP); Tg(ins:dsRed) larvae stained for glucagon (red). (E) Quantification of gcga:GFP+ α cells and ins+ β cells in control MO-injected (n=15) and gcgaMO-injected (n=10) islets. (F) Quantification of H2B-RFP+ and H2B-RFP β cells at 3 and 5dpf in control MO-injected (ne3) and gcgaMO-injected (n=5) islets. Ventral bud-derived H2B-RFP β cells are specifically diminished in gcga morphants. *P≤0.05, **P≤0.01, ***P≤0.001; ns, not significant (two-way ANOVA followed by Bonferroni post-test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed ia1Tg; m1018Tg control Day 5 pancreatic B cell IFL
ia1Tg; m1018Tg + MO1-gcga standard conditions Day 5 pancreatic B cell IFL
gcga WT control Day 4 intestinal bulb RTPCR
Day 4 liver RTPCR
Day 4 pancreas RTPCR
Day 5 intestinal bulb RTPCR
Day 5 liver RTPCR
Day 5 pancreas RTPCR
Days 7-13 intestinal bulb RTPCR
Days 7-13 liver RTPCR
Days 7-13 pancreas RTPCR
WT chemical treatment: pharmaceutical Day 4 intestinal bulb RTPCR
Day 4 liver RTPCR
Day 4 pancreas RTPCR
Day 5 intestinal bulb RTPCR
Day 5 liver RTPCR
Day 5 pancreas RTPCR
Days 7-13 intestinal bulb RTPCR
Days 7-13 liver RTPCR
Days 7-13 pancreas RTPCR
ia1Tg; m1018Tg control Day 5 pancreatic A cell IHC
ia1Tg; m1018Tg + MO1-gcga standard conditions Day 5 Not Detected pancreatic A cell IHC
GFP ia1Tg; m1018Tg control Day 5 pancreatic A cell IFL
ia1Tg; m1018Tg + MO1-gcga standard conditions Day 5 pancreatic A cell IFL
ins WT control Day 4 intestinal bulb RTPCR
Day 4 liver RTPCR
Day 4 pancreas RTPCR
Day 5 intestinal bulb RTPCR
Day 5 liver RTPCR
Day 5 pancreas RTPCR
Days 7-13 intestinal bulb RTPCR
Days 7-13 liver RTPCR
Days 7-13 pancreas RTPCR
WT chemical treatment: pharmaceutical Day 4 Not Detected intestinal bulb RTPCR
Day 4 Not Detected liver RTPCR
Day 4 Not Detected pancreas RTPCR
Day 5 intestinal bulb RTPCR
Day 5 liver RTPCR
Day 5 pancreas RTPCR
Days 7-13 intestinal bulb RTPCR
Days 7-13 liver RTPCR
Days 7-13 pancreas RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development