FIGURE

Fig. 3

ID

ZDB-FIG-150507-2

Publication

Benini et al., 2015 - slc7a6os Gene Plays a Critical Role in Defined Areas of the Developing CNS in Zebrafish

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Fig. 3

Morphological analysis of slc7a6os morphants and phenotypic rescue at 28 h.

Embryos were injected at the two-cells stage with 6 ng/embryo of slc7a6os-MOspl1 splicing-inhibiting morpholino and examined at different developmental stages. At 28 hpf MO injected embryos exhibit alterations in somites structure and brain malformations (B, E, H) when compared to control embryos (A, D, G). Magnification of lateral views show undefined boundaries between brain subregions, especially at hindbrain and midbrain regions (H). The morphological differences observed in morphant embryos are rescued by expression of synthetic slc7a6os mRNA (C, F, I). Abbreviations: h, hindbrain; c, cerebellum; m, midbrain; MHB, midbrain-hindbrain boundary; so, somites.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-slc7a6os
Knockdown Reagent:	MO1-slc7a6os
Observed In:	central nervous system somite whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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