FIGURE

Fig. 2

ID

ZDB-FIG-150427-51

Publication

Liu et al., 2015 - Evolutionarily conserved regulation of hypocretin neuron specification by Lhx9

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Fig. 2

Examples of expression patterns of Hcrt neuron-enriched genes. (A,C,E) ISH performed on 24hpf zebrafish embryos using probes specific for hcrt, hmx2 and lhx9. Dashed boxes indicate the hypothalamic regions shown in B,D,F. (B,D,F) A 1.25-µm confocal section of fluorescent ISH (red) and hcrt:EGFP immunofluorescence (green). All hcrt:EGFP neurons express hmx2 and lhx9. hmx2 expression extends ventrally (C,D) and lhx9 is expressed in broader domains of the telencephalon and diencephalon (E,F), as well as the hindbrain and spinal cord (not shown). Supplementary material Movie 1 contains the complete confocal image stack for F. (G) Mean±s.e.m. number of cells in the boxed regions in A,C,E. n, number of brains. Scale bars: 10µm.

Expression Data

Genes:	EGFP hcrt hmx2 lhx9
Fish:	WT zf11Tg
Anatomical Terms:	brain hypocretin-secreting neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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