FIGURE

Fig. S11

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ZDB-FIG-150427-66

Publication

Liu et al., 2015 - Evolutionarily conserved regulation of hypocretin neuron specification by Lhx9

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Fig. S11

Endogenous hcrt- and pdyn-expressing neurons are reduced in lhx9 morphants. Tg(hcrt:EGFP) embryos injected with either lhx9 morpholino (C, D) or lhx9 mismatch control morpholino (A, B) were fixed at 72 hpf and probed for vglut1 or pdyn expression by fluorescent ISH. No obvious defects in vglut1 expression were observed in lhx9 morphants, but the total number of cells with intense, punctate pdyn expression was reduced by approximately 40% (E). This result suggests that the morpholino-induced reduction in Hcrt neurons is caused by cell loss, rather than silencing of hcrt expression. n indicates number of morphant brains analyzed. ***, p<0.001 compared to embryos injected with the control morpholino by Student’s t-test. Scale = 10 µm.

Expression Data

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Antibody Labeling

Phenotype Data

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