FIGURE

Fig. 2

ID

ZDB-FIG-150408-39

Publication

Shiau et al., 2015 - Differential Requirement for irf8 in Formation of Embryonic and Adult Macrophages in Zebrafish

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Fig. 2

irf8 mutant embryos have no macrophages but produce excessive neutrophils.

(A) mfap4 RNA expression at 2 dpf shows a complete loss of macrophages in irf8 mutant but abundant macrophages in heterozygous sibling (arrows). (B) mpx RNA expression at 2 dpf shows an overproduction of neutrophils in irf8 mutants (arrows, bottom panels) compared with the sibling (arrow, top panels). Right column in A and B shows a higher magnification image of the trunk region from the same embryos depicted in the left column. (C) Neutrophils can first be detected on the yolk sac by 1 dpf. irf8 mutants have many yolk sac neutrophils (arrows) compared with sibling. (D) At 2 dpf, irf8 mutants continue to have more neutrophils on the yolk sac (arrows). Overall, irf8 mutants appear to have more neutrophils throughout the body. All scale bars are 100 um.

Expression Data

Genes:	mfap4.1 mpx
Fish:	WT irf8^st95/st95
Anatomical Terms:	macrophage neutrophil
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	irf8^st95/st95
Observed In:	macrophage neutrophil
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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