FIGURE

Fig. 3

ID

ZDB-FIG-150408-30

Publication

Merkes et al., 2015 - Ewing Sarcoma Ewsa Protein Regulates Chondrogenesis of Meckel's Cartilage through Modulation of Sox9 in Zebrafish

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Fig. 3

Prehypertrophic chondrocytes of Meckel’s cartilage in MZ ewsa/ewsa fails to differentiate into hypertrophic chondrocytes.

A. Flat mounted Meckel’s cartilage of 4 dpf (left) wt/wt and (right) MZ ewsa/ewsa. B. Number of cells of Meckel’s cartilage of 4 dpf wt/wt and MZ ewsa/ewsa zebrafish (P = 0.0002). C. Lateral views (anterior to the left, dorsal to the top) of 4dpf (Left) wt/wt and (Right) MZ ewsa/ewsa visualized by in situ hybridization using probe for ihha (top panel) and colX (bottom panel). m: Meckel′s cartilage. D. Ventral views (anterior to the left) of Meckel′s cartilage of (Left) wt/wt and (Right) MZ ewsa/ewsa visualized by immunohistochemistry using (top) anti-IHH antibody (green), and anti-Collagen type II antibody (Red) (4dpf), and (bottom) anti-Collagen X antibody (green) and anti-Collagen type II antibody (Red) (5dpf).

Expression Data

Genes:	col10a1a ihha
Antibodies:	Ab1-col10a1 Ab1-ihh
Fish:	AB ewsr1a^{zm00260171Tg/zm00260171Tg}
Anatomical Terms:	hypertrophic chondrocyte Meckel's cartilage chondrocyte Meckel's cartilage hypertrophic chondrocyte
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ewsr1a^{zm00260171Tg/zm00260171Tg}
Observed In:	chondrocyte hypertrophy Meckel's cartilage chondrocyte
Stage:	Day 4

Phenotype Detail

Acknowledgments

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