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ZFIN ID: ZDB-FIG-150407-5
Renz et al., 2015 - Regulation of β1 Integrin-Klf2-Mediated Angiogenesis by CCM Proteins. Developmental Cell   32:181-190 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 5

Blood-Flow-Independent Expression of klf2a/b mRNA in ccm2m201 Mutants

(A–C) The ccm2m201 mutant cardiac ballooning phenotype is flow independent. Frontal views of 48 hrpf hearts of the myocardial reporter line Tg(myl7:GFP)twu34 (Huang et al., 2003) show that cardiac expansion is not rescued in ccm2m201 mutant; tnnt2a morphants that lack blood flow.

(D) The expression levels of klf2a and klf2b mRNA, as measured by qRT-PCR, are elevated in ccm2m201 mutant; tnnt2a morphants that lack blood flow.

(E–G) Loss of Ccm2 results in overproliferation of the endocardium under no-flow conditions. (E) Quantifications of the ratio of proliferating versus nonproliferating endocardial cells between 33 and 53 hrpf in tnnt2a morphants compared with ccm2m201 mutant;tnnt2a morphants based on SPIM recordings (n = 2). (F) The endocardium of tnnt2a morphants is not proliferative as indicated by the lack of color-coded proliferative cells (still image derived from Movie S1, which is based on SPIM time-lapse recordings of endocardial morphogenesis marked by Tg(kdrl:GFP)s843 between 33 and53 hrpf). (G) The combined loss of Ccm2 and Tnnt2a is characterized by a high rate of proliferative events (indicated by uniquely colored daughter cells derived from proliferative events; still image derived from Movie S2). A, atrium; V, ventricle. Statistical data are means and SEM; ns, not significant; p < 0.05; p < 0.01; p < 0.001; p < 0.0001; see  Supplemental Experimental Procedures for details about statistical data analysis.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ccm2m201/m201; twu34Tg standard conditions Long-pec cardiac muscle IFL
ccm2m201/m201; twu34Tg + MO1-tnnt2a standard conditions Long-pec cardiac muscle IFL
twu34Tg standard conditions Long-pec cardiac muscle IFL
klf2a WT standard conditions Long-pec whole organism RTPCR
WT + MO1-tnnt2a standard conditions Long-pec whole organism RTPCR
ccm2m201/m201 standard conditions Long-pec whole organism RTPCR
ccm2m201/m201 + MO1-tnnt2a standard conditions Long-pec whole organism RTPCR
klf2b WT standard conditions Long-pec whole organism RTPCR
WT + MO1-tnnt2a standard conditions Long-pec whole organism RTPCR
ccm2m201/m201 standard conditions Long-pec whole organism RTPCR
ccm2m201/m201 + MO1-tnnt2a standard conditions Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ccm2m201/m201; s843Tg + MO1-tnnt2a standard conditions Prim-5 - Long-pec endocardium proliferative, abnormal
ccm2m201/m201; twu34Tg standard conditions Long-pec heart edematous, abnormal
Long-pec heart increased size, abnormal
ccm2m201/m201; twu34Tg + MO1-tnnt2a standard conditions Long-pec heart edematous, abnormal
ccm2m201/m201; twu34Tg + MO1-tnnt2a standard conditions Long-pec heart increased size, abnormal
s843Tg + MO1-tnnt2a standard conditions Prim-5 - Long-pec endocardium hypotrophic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 32, Renz, M., Otten, C., Faurobert, E., Rudolph, F., Zhu, Y., Boulday, G., Duchene, J., Mickoleit, M., Dietrich, A.C., Ramspacher, C., Steed, E., Manet-Dupé, S., Benz, A., Hassel, D., Vermot, J., Huisken, J., Tournier-Lasserve, E., Felbor, U., Sure, U., Albiges-Rizo, C., Abdelilah-Seyfried, S., Regulation of β1 Integrin-Klf2-Mediated Angiogenesis by CCM Proteins, 181-190, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell