FIGURE

Fig. 3

ID

ZDB-FIG-150406-28

Publication

Boer et al., 2015 - Fascin1-Dependent Filopodia are Required for Directional Migration of a Subset of Neural Crest Cells

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Fig. 3

fscn1a is required for filopodia formation in NC cells.

(A) Dorsal cranial views of cranial NC streams in 26 hpf Tg(sox10:rfpmb) and Tg(sox10:rfpmb); fscn1a MZ embryos, anterior is to the left. Numbers correspond to pharyngeal arches, e = eye, ov = otic vesicle, nt = neural tube, scale bar = 100µm. Leading edge of NC stream 1 is enlarged in right panel, scale bar = 10 µm. Arrows highlight filopodia at leading edge of NC stream 1. (B-C) Time lapse confocal images of filopodia at the leading edge of NC stream 2 (B) or of leading cells in trunk NC streams (C) in live Tg(sox10:rfpmb) and Tg(sox10:rfpmb); fscn1a MZ embryos at 26 hpf. Arrows highlight tips of single filopodia throughout the time lapses. Scale bar = 10 µm. (D) Quantitation of filopodia number, mean and maximum filopodia length, and change in filopodia length in Tg(sox10:rfpmb) and Tg(sox10:rfpmb); fscn1a MZ cranial NC (n = 6 embryos and 30 filopodia each for Tg(sox10:rfpmb) and Tg(sox10:rfpmb); fscn1a MZ, ****p<0.0001).

Expression Data

Gene:	RFP
Fish:	vu234Tg fscn1a^{zd1012/zd1012}; vu234Tg
Anatomical Terms:	ectomesenchyme filopodium membrane neural crest filopodium membrane neural crest membrane trunk neural crest filopodium membrane
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fscn1a^{zd1012/zd1012}; vu234Tg
Observed In:	cranial neural crest filopodium
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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