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ZFIN ID: ZDB-FIG-150401-18
Xu et al., 2014 - Maternal Vsx1 plays an essential role in regulating prechordal mesendoderm and forebrain formation in zebrafish. Developmental Biology   394(2):264-76 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Bud
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Bud

Fig. 5

Impaired PME formation and progression resulted in aberrant forebrain formation. (A, D, G) Phenotype comparison of uninjected (A), vsx1 tbMO injected (D) and vsx1 tbMO and dkk1 mRNA coinjected embryos (G) at 8-10 somite stage. Brackets show the distance between head and tail. Note that the forebrain formation is restored with a shortened AP axis and anterior neural plate marker is expressed in more posterior positions in vsx1 tbMO and dkk1 mRNA coinjected embryos. (B-C′, E-F′, H, H′) Whole-mount in situ hybridization of anterior neuroectoderm marker rx3 and PME marker hgg1 at bud stage. (B, C, E, F, H) Dorsal-animal views with anterior to the top. (B′, C′, E′, F′, H′) Lateral views with animal to the top. (I) Proportion of brain phenotypes in different experiments at 24 hpf. The injected reagents and their amounts are indicated at the bottom. Numbers of examined embryos are indicated at the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT standard conditions Bud prechordal plate ISH
WT + MO2-vsx1 standard conditions Bud prechordal plate ISH
rx3 WT standard conditions Bud forebrain neural plate ISH
WT + MO2-vsx1 standard conditions Bud forebrain neural plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-vsx1 standard conditions Bud forebrain development process quality, abnormal
Bud prechordal plate formation process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 394(2), Xu, X., He, Y., Sun, L., Ma, S., Luo, C., Maternal Vsx1 plays an essential role in regulating prechordal mesendoderm and forebrain formation in zebrafish, 264-76, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.