FIGURE

Fig. 11

ID

ZDB-FIG-150318-5

Publication

Rajaram et al., 2014 - Dynamic miRNA Expression Patterns During Retina Regeneration in Zebrafish: Reduced Dicer or miRNA Expression Suppresses Proliferation of Müller Glia-Derived Neuronal Progenitor Cells

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Fig. 11

Knockdown of miR-2190 does not affect proliferation. Adult albino zebrafish were intravitreally injected and electroporated with either standard control or miR-2190 morpholino prior to the start of light damage. A,B: After 51h of constant light, standard control and miR-2190 morphant retinas both display PCNA-positive doublet INL proliferating nuclei (arrows). C,D: After 72h of light, standard control and miR-2190 morphant retinas display clusters of INL nuclei (arrowheads). E,F: Statistically equivalent numbers of INL and ONL PCNA-positive nuclei are present in standard control and miR-2190 morphant retinas. INL, inner nuclear layer; ONL, outer nuclear layer. Scale bar in A = 50 µm and applies to B–D; data represent mean ± s.e.m. P > 0.05 using two-way ANOVA with Tukey′s post-hoc test, n=6.

Expression Data

Antibody:	Ab2-pcna
Fish:	slc45a2^b4/b4 slc45a2^b4/b4 + MO1-mir2190
Condition:	constant light, high light intensity, light damage: retina
Knockdown Reagent:	MO1-mir2190
Anatomical Terms:	retinal inner nuclear layer retinal outer nuclear layer
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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