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Knockdown of miR-142b reduces proliferation. Adult albino zebrafish were intravitreally injected and electroporated with either standard control or miR-142b morpholino prior to the start of light damage. A,B: After 51h of constant light, doublet PCNA-positive INL cells (arrows) were present in both the standard control and miR-142b morphant retinas. C,D: After 72h, PCNA-positive INL cell clusters are present in standard control morphants (C, arrowhead), but only doublet or fewer PCNA-positive INL cells (D, arrow) remained in miR-142b morphant retinas. E,F: miR-142b knockdown significantly reduced the number of PCNA-positive INL cells at both 51 and 72h and ONL cells at 72h. INL, inner nuclear layer; ONL, outer nuclear layer. Scale bar in A = 50 µm and applies to B–D; data represent mean ± s.e.m. *P< 0.05 using two-way ANOVA with Tukey′s post-hoc test, n=6.
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