FIGURE

Fig. 4

ID

ZDB-FIG-150313-25

Publication

De Smet et al., 2014 - Fibroblast Growth Factor Signaling Affects Vascular Outgrowth and Is Required for the Maintenance of Blood Vessel Integrity

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Fig. 4

Role of FGFs in Other Angiogenesis Processes in Zebrafish

(A and B) Fluorescent images (ventral view) of GFP⁺ subintestinal vessels (SIVs) on the surface of the yolk sac of 72 hpf Tg(fli1:EGFP)^y1 zebrafish embryos, revealing normal SIVs after exposure to DMSO (A) and severe underdevelopment of these SIVs after exposure to 50 μM SSR (tissue concentration 1.9 μg SSR/mg protein) from 20 hpf onward (arrowheads in B).

(C and D) Images (lateral view) of the GFP⁺ vascular plexus (VP) in the posterior trunk of 45 hpf Tg(fli1:EGFP)^y1 zebrafish embryos. In DMSO treated control embryos (C), a vascular plexus formed via branching from the caudal vein. After exposure to 200 μM SSR (tissue concentration 3.9 μg SSR/mg protein) from 20 hpfs onward, the vascular plexus failed to remodel and branch and, instead, only a large vascular syncytium was formed (D). In all panels, the head of the embryo faces left; scale bars denote 50 μm in (A) and (B) and 100 μm in (C) and (D).

Expression Data

Gene:	EGFP
Fish:	y1Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Terms:	caudal vein plexus subintestinal vein
Stage Range:	High-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg
Condition:	chemical treatment: pharmaceutical
Observed In:	caudal vein plexus caudal vein plexus blood vessel development subintestinal vein subintestinal vein blood vessel development
Stage Range:	High-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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