PUBLICATION
Fibroblast Growth Factor Signaling Affects Vascular Outgrowth and Is Required for the Maintenance of Blood Vessel Integrity
- Authors
- De Smet, F., Tembuyser, B., Lenard, A., Claes, F., Zhang, J., Michielsen, C., Van Schepdael, A., Herbert, J.M., Bono, F., Affolter, M., Dewerchin, M., Carmeliet, P.
- ID
- ZDB-PUB-140910-5
- Date
- 2014
- Source
- Chemistry & Biology 21(10): 1310-7 (Journal)
- Registered Authors
- Affolter, Markus, Lenard, Anna
- Keywords
- none
- MeSH Terms
-
- Signal Transduction/drug effects
- Regeneration
- ortho-Aminobenzoates/chemistry
- ortho-Aminobenzoates/metabolism
- ortho-Aminobenzoates/pharmacology
- Animals
- Indolizines/chemistry
- Indolizines/metabolism
- Indolizines/pharmacology
- Intercellular Junctions/metabolism
- Embryo, Nonmammalian/metabolism
- Receptors, Fibroblast Growth Factor/antagonists & inhibitors
- Receptors, Fibroblast Growth Factor/metabolism*
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/metabolism*
- Blood Vessels/drug effects
- Blood Vessels/metabolism*
- Animal Fins/physiology
- Zebrafish/growth & development
- Zebrafish/metabolism
- Cadherins/metabolism
- Neovascularization, Physiologic/drug effects
- PubMed
- 25200605 Full text @ Chem. Biol.
Citation
De Smet, F., Tembuyser, B., Lenard, A., Claes, F., Zhang, J., Michielsen, C., Van Schepdael, A., Herbert, J.M., Bono, F., Affolter, M., Dewerchin, M., Carmeliet, P. (2014) Fibroblast Growth Factor Signaling Affects Vascular Outgrowth and Is Required for the Maintenance of Blood Vessel Integrity. Chemistry & Biology. 21(10):1310-7.
Abstract
Angiogenesis contributes to the development of numerous disorders. Even though fibroblast growth factors (FGFs) were discovered as mediators of angiogenesis more than 30 years ago, their role in developmental angiogenesis still remains elusive. We use a recently described chemical probe, SSR128129E (SSR), that selectively inhibits the action of multiple FGF receptors (FGFRs), in combination with the zebrafish model to examine the role of FGF signaling in vascular development. We observe that while FGFR signaling is less important for vessel guidance, it affects vascular outgrowth and is especially required for the maintenance of blood vessel integrity by ensuring proper cell-cell junctions between endothelial cells. In conclusion, our work illustrates the power of a small molecule probe to reveal insights into blood vessel formation and stabilization and thus of broad interest to the vascular biology community.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping