FIGURE

Fig. 7

ID

ZDB-FIG-150311-63

Publication

Li et al., 2014 - Ncor1 and Ncor2 Play essential but distinct roles in zebrafish primitive myelopoiesis

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Fig. 7

Overexpressing tal1/lmo2 mRNA could rescue the depleted myeloid cells in ncor2 or ncor1 morphants at 24 hpf. A–C: The expression of mfap4 was examined by whole-mount in situ hybridization. D–F,J–L: The expression of mpx was examined by whole-mount in situ hybridization. G–I: Whole-mount in situ hybridization was performed to examine the expression of spi1b. Embryos were microinjected with control MO (A,D,G,J), ncor2 MO (B,E,H), ncor2 MO plus tal1/lmo2 mRNA (C,F,I), ncor1 MO (K) and ncor1 MO plus tal1/lmo2 mRNA (L), respectively. M–O: Scatter plots showing the number of mfap4⁺ (M), mpx⁺ (N), or spi1b⁺ (O) cells in the embryos microinjected with control MO, ncor2 MO, and tal1/lmo2 mRNA plus ncor2 MO, respectively. P: Scatter plot showing the number of mpx⁺ cells in the embryos microinjected with control MO, ncor1 MO, and tal1/lmo2 mRNA plus ncor1 MO, respectively.

Expression Data

Genes:	mfap4.1 mpx spi1b
Fish:	WT WT + MO1-ncor1 WT + MO2-ncor2
Knockdown Reagents:	MO1-ncor1 MO2-ncor2
Anatomical Terms:	macrophage neutrophil rostral blood island
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ncor1 WT + MO2-ncor2
Knockdown Reagents:	MO1-ncor1 MO2-ncor2
Observed In:	macrophage neutrophil
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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