FIGURE

Fig. 4

ID

ZDB-FIG-150311-60

Publication

Li et al., 2014 - Ncor1 and Ncor2 Play essential but distinct roles in zebrafish primitive myelopoiesis

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Fig. 4

Zebrafish ncor2 but not ncor1 is essential for the development of spi1b⁺ cells in the embryos at 10-somite stage. A–C,A2–C2: Whole-mount in situ hybridization was performed (myoD: a marker to indicate the developmental stage) to examine the expressions of spi1b. Embryos were microinjected with control MO (A,A2), ncor2 MO (B,B2) and ncor1 MO (C,C2) respectively. Embryos were positioned anterior top and viewed laterally (A–C) or anterior left and viewed dorsally (A2–C2). Arrowhead indicates the decreased expression of spi1b in the RBI (B,B2). The number shown in the lower right-hand corner is the number of embryos exhibiting the typical phenotype shown in the panel to the number of embryos totally observed. D: qPCR was performed to confirm the changes of expression levels of spi1b in ncor2 and ncor1 morphants. Experiments were repeated three times. *P < 0.05.

Expression Data

Gene:	spi1b
Fish:	WT WT + MO1-ncor1 WT + MO2-ncor2
Knockdown Reagents:	MO1-ncor1 MO2-ncor2
Anatomical Term:	rostral blood island
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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