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ZFIN ID: ZDB-FIG-150204-626
Wu et al., 2014 - Nuclear Receptor Subfamily 2 Group F Member 1a (nr2f1a) Is Required for Vascular Development in Zebrafish. PLoS One   9:e105939 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5

Fig. 8

nr2f1a modulates the expression of vascular markers.

(A-H) Compared to wild type controls (A, C, E, G), expression of the venous markers mrc1 (D) and flt4 (F) were reduced in the trunk of nr2f1a morphants at 24 hpf. In addition, expression of the arterial marker ephrinb2 (H) and pan-vascular marker stabilin decreased (B). (I) Quantification of the relative expression level by qPCR assay showed a ~25% to 50% reduced expression in vascular markers flt4 (0.66±0.14), EphB4 (0.46±0.08), gridlock (0.73±0.06) and ephrinb2 (0.59±0.05) in nr2f1a morphants. *** refers to p<0.0001, ** refers to p<0.001 and * refers to p<0.05 by an unpaired student’s t-test. Data are represented as means ± SEM. Scale bars represent 100 µm in A-H.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ephb4a AB control Prim-5 trunk vasculature artery ISH
AB + MO3-nr2f1a standard conditions Prim-5 trunk vasculature artery ISH
flt4 AB control Prim-5 trunk vasculature vein ISH
AB + MO3-nr2f1a standard conditions Prim-5 trunk vasculature vein ISH
mrc1a AB control Prim-5 trunk vasculature vein ISH
AB + MO3-nr2f1a standard conditions Prim-5 trunk vasculature vein ISH
stab2 AB control Prim-5 trunk vasculature ISH
AB + MO3-nr2f1a standard conditions Prim-5 trunk vasculature ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One