Fig. 5

Loss of nr2f1a results in circulation defects and pericardial edema.

(A-E) nr2f1a^e2i2 MO was injected into transgenic Tg (fli:eGFP^y1; gata1:dsRed) embryos with GFP-labeled endothelial cells (A, C) and dsRed-labeled blood cells (B, D, E). Loss of nr2f1a showed a mis-pattern at ISV and at the caudal vein plexus (CVP) and results in circulation defects in severe (D, no circulation and blood stock) or mild (E, blood stock at CVP and shorter circulation) at 52 hpf compared to wild-type fish (B). (F) Circulation defects at the ISV/DLAV (~68% in morphants) and slow to lose axial circulation of the aorta/vein in the trunk region (~58% in morphants) are quantitated in wt (n = 37) and nr2f1aMO (n = 42) at 48-52 hpf. (G, H) Representative edema fish and quantitative results from three independent experiments showed 65% of nr2f1a morphants (n = 69) with mild to severe pericardial edema compared to wt (n = 30). The scale bar in A-E represent 200 µm and in G is 500 µm.