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ZIRC
ZFIN ID: ZDB-FIG-141007-100
Li et al., 2014 - Overexpression of miR-19b Impairs Cardiac Development in Zebrafish by Targeting ctnnb1. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology   33:1988-2002 Full text @ Cell Physiol. Biochem.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Long-pec

Fig. 10

MiR-19b directly targets ctnnb1. A representative image of ctnb1 expression in 48 hpf embryos determined by in situ hybridization is shown (A). The expression in the miR-19b mimic-injected embryos was decreased when compared with WT and NC-injected embryos. Similarly, real-time PCR showed the expression level of ctnnb1 at 24 and 48 hpf was significantly downregulated in the mir-19b mimic-injected embryos. A schematic of the luciferase vector-expressing ctnnb1 32UTR construct is shown (C). Luciferase assay with ctnnb1 32UTR of WT, NC and miR-19b mimic-injected embryos is presented (D). ** P < 0.01; *** P < 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctnnb1 WT control Long-pec heart ISH
WT standard conditions Long-pec heart ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Physiol. Biochem.