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Fig. 10
MiR-19b directly targets ctnnb1. A representative image of ctnb1 expression in 48 hpf embryos determined by in situ hybridization is shown (A). The expression in the miR-19b mimic-injected embryos was decreased when compared with WT and NC-injected embryos. Similarly, real-time PCR showed the expression level of ctnnb1 at 24 and 48 hpf was significantly downregulated in the mir-19b mimic-injected embryos. A schematic of the luciferase vector-expressing ctnnb1 32UTR construct is shown (C). Luciferase assay with ctnnb1 32UTR of WT, NC and miR-19b mimic-injected embryos is presented (D). ** P < 0.01; *** P < 0.001.