FIGURE

Fig. 2

ID

ZDB-FIG-140917-8

Publication

Weicksel et al., 2014 - Targeted germ line disruptions reveal general and species-specific roles for paralog group 1 hox genes in zebrafish

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Fig. 2

Zebrafish hoxb1b is required for hindbrain segmentation. A-O. Wild type (A, E, I, M), hoxb1a^-/-(B, F, J, N), hoxb1b^-/-(C, G, K, O) or doubly hoxb1a^-/-;hoxb1b^-/- embryos (D, H, L) were assayed by in situ hybridization for expression of hoxb1a in r4 (blue stain in A-D), krox20 in r3/r5 (red stain in A-D, I-O and blue stain in panels E-H), pax2 at the midbrain-hindbrain boundary (blue stain in E-H), fgf3 in r4 (blue stain in I-L), hoxd4a in r7 (blue stain in E-H) and hoxb3a in r5/r6 (blue stain in M-O). P. Quantification of segmentation defects in hoxb1b^-/- embryos. Rhombomere lengths were measured as indicated in the inset. MHB-r6 measures the full distance from the anterior limit of the MHB to the posterior limit of r6. p-values were computed using Students’ t-test and error bars represent standard error. N = 10 embryos. All embryos are flat mounted in dorsal view with anterior to the top. A-H and M-O are at 22hpf, while I-L are at 14hpf. r = rhombomere; MHB = midbrain/hindbrain boundary.

Expression Data

Genes:	egr2b fgf3 hoxb1a hoxb3a hoxd4a pax2a
Fish:	WT hoxb1b^um197/um197 hoxb1a^um191/+; hoxb1a^um192/+ hoxb1a^um193/+; hoxb1a^um194/+; hoxb1b^um197/um197
Anatomical Terms:	midbrain hindbrain boundary rhombomere 3 rhombomere 4 rhombomere 5 rhombomere 6 rhombomere 7 rhombomere 8
Stage Range:	10-13 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hoxb1b^um197/um197 hoxb1a^um193/+; hoxb1a^um194/+; hoxb1b^um197/um197
Observed In:	hindbrain rhombomere 3 rhombomere 4 rhombomere 6
Stage:	26+ somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.