FIGURE

Fig. 4

ID

ZDB-FIG-140909-36

Publication

Rong et al., 2014 - R-Spondin 3 Regulates Dorsoventral and Anteroposterior Patterning by Antagonizing Wnt/β-Catenin Signaling in Zebrafish Embryos

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Fig. 4

Zebrafish Rspo3 regulates dorsoventral patterning.

(A) Expression patterns of the indicated marker genes in wild-type (WT) embryos or embryos injected with 4 ng control MO (cMO), 4 ng rspo3 targeting MO1 or 600 pg rspo3 mRNA, respectively. Embryos were analyzed at the shield stage by in situ hybridization using the indicated probes. Panels a–d and i-p are top views with animal pole up and dorsal to the right; panels e–h are lateral and dorsal views with animal pole up. Arrows indicate the width of the expression domains; asterisks indicate the edges of the expression domains. Percentages of embryos in each category were calculated and are shown in B (chd), C (gsc), D (eve1) and E (ved). The total embryo numbers from three independent experiments are shown on the top of each bar. Scale bar = 200 μm.

Expression Data

Genes:	chrd eve1 gsc ved
Fish:	WT WT + MO1-rspo3
Knockdown Reagent:	MO1-rspo3
Anatomical Terms:	presumptive mesoderm ventral region shield
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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