FIGURE

Fig. 8

ID

ZDB-FIG-140822-46

Publication

Stegmaier et al., 2014 - Fast segmentation of stained nuclei in terabyte-scale, time resolved 3D microscopy image stacks

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Fig. 8

Results of the TWANG segmentation pipeline applied on two images of a developing zebrafish embryo.

The images were captured at the 7≈8000 cells (A,B) and at the 11 hpf stage with ≈12000 cells (C,D), respectively. The panels show maximum intensity projections of the raw images (A,C) and the resulting segmentation using our TWANG segmentation pipeline (B,D). Each 3D image stack has a file size of ~5GB and comprises 2560×2160×500 voxels with a dynamic range of 16 bits. Processing one image stack takes approximately 10-20 minutes on a common desktop machine, depending on the developmental stage of the embryo. Typical experiments may be comprised of up to 2000 z-stacks (~10TB) for the spatio-temporal analysis of a single embryo.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One