FIGURE

Fig. 6

ID

ZDB-FIG-140804-18

Publication

Forn-Cuní et al., 2014 - The Evolution and Appearance of C3 Duplications in Fish Originate an Exclusive Teleost c3 Gene Form with Anti-Inflammatory Activity

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Fig. 6

Developmental and inflammatory effects of the c3.1 and c3.7/8 inhibition.

(A) MO-c3.1s injected at high concentrations produced aberrant phenotypes and increased mortality. Co-injecting with c3.1 capped mRNA recovered the WT phenotype in a dose-dependent manner (LD: 2,25 ng c3.1 capped RNA; HD: 4,5 ng c3.1 capped RNA). (B) Representative individuals of the c3.1 inhibition treatments and its phenotypic rescue. (C) MO-c3.7/8s microinjected larvae showed a major migration number of neutrophils to the amputation site 3 h after tail clipping (confocal images). Statistical significance was determined by unpaired T-tests (**P<0.01, ***P<0.001). (D) MO-c3.7/8s microinjected larvae significantly expressed higher levels of il1b 3 h after bath infection with A. hydrophila.

Expression Data

Gene:	il1b
Fish:	WT WT + MO2-c3b
Condition:	bacterial treatment: Aeromonas hydrophila
Knockdown Reagent:	MO2-c3b
Anatomical Term:	whole organism
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-c3a.1 i114Tg + MO2-c3b
Knockdown Reagents:	MO1-c3a.1 MO2-c3b
Observed In:	embryo development neutrophil migration whole organism
Stage Range:	Long-pec to Adult

Phenotype Detail

Acknowledgments

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