FIGURE

Fig. S8

ID

ZDB-FIG-140801-6

Publication

Coxam et al., 2014 - Pkd1 Regulates Lymphatic Vascular Morphogenesis during Development

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Fig. S8

No evidence for a contribution of primary cilia to lymphangiogenesis. Related to Discussion section.

(A-B) The vasculature Tg(lyve1:DsRed2)^nz101

in (A) wild-type sibling and (B) ift88^tz288 mutant embryos at 56 hpf (arrowheads indicate parachordal lymphangioblasts and white arrows indicate venous sprouts).

(C-D) Overall morphology of (C) wild-type siblings and (D) ift88^tz288 mutants at 5 dpf. (E-H) The vasculature Tg(lyve1:DsRed2^nz101; flt1:YFP^hu4624Tg) of (E,G) WT and (F,H) ift88^tz288 mutants at 5 dpf.

(I-J) Quantification of (I) secondary sprouts in WT (n=12) and ift88^tz288 (n=10) at 56 hpf and (J) thoracic duct extent in WT (n=16) and ift88tz288 (n=18).

(K-N) Overview of primary cilia localization in the trunk of (K,M) WT (n=4) and (L,N) lyc1 mutants (n=3) embryos at 30 hpf, stained for blood vessels (Kdrl-Cherry), nuclei (DAPI) and primary cilia (Acetylatedtubulin) markers. Arrowheads indicate example of discrete primary cilia. (O) An individual representative primary cilium in a lyc1 embryo.

(P) Transient expression of a Pkd1-YFP BAC construct in an arterial intersegmental vessel and adjacent muscle cells at 4 dpf. DA: Dorsal Aorta, PCV: Posterior Cardinal Vein, TD: Thoracic duct

Expression Data

Genes:	DsRed YFP
Fish:	nz101Tg hu4624Tg; nz101Tg ift88^tz288/tz288; hu4624Tg; nz101Tg ift88^tz288/tz288; nz101Tg
Anatomical Terms:	dorsal aorta intersegmental vessel posterior cardinal vein thoracic duct trunk vasculature vascular lymphangioblast vascular sprouts
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ift88^tz288/tz288
Observed In:	whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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