FIGURE

Fig. 1

ID

ZDB-FIG-140801-1

Publication

Coxam et al., 2014 - Pkd1 Regulates Lymphatic Vascular Morphogenesis during Development

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Fig. 1

lyc1 Mutants Display Reduced Lymphatic Development

(A and B) Overall morphology of wild-type siblings (A) and lyc1 mutants (B) at 4 dpf.

(C and D) The vasculature Tg(fli1a:EGFP^y1; flt1:tomato^hu5333Tg) of (C) wild-type (WT) (arrowheads indicate thoracic duct) and (D) lyc1 mutants at 4 dpf (asterisks indicate absence of thoracic duct).

(E and G) The vasculature Tg(fli1a:EGFP^y1; flt1:tomato^hu5333Tg) in wild-type sibling (E) and mutant embryos (G) at 56 hpf (arrows indicate lymphatic precursors known as parachordal lymphangioblasts, PLs).

(F and H) flt1:tomato^hu5333Tg expression marks the arterial ECs, a loss of signal (brackets) indicating venous intersegmental vessels (vISVs).

(I–K) Quantification of (I) thoracic duct extent across ten somites (WT n = 40, lyc1 n = 17), (J) parachordal lymphangioblasts (WT n = 78, lyc1 n = 17), and (K) venous sprouts (WT n = 40, lyc1 n = 15). DA, dorsal aorta; PCV, posterior cardinal vein.

Error bars indicate SEM. See also Figures S1 and S2.

Expression Data

Genes:	EGFP RFP
Fish:	hu5333Tg; y1Tg pkd1a^{hu5855/hu5855}; hu5333Tg; y1Tg
Anatomical Terms:	artery dorsal aorta intersegmental vein intersegmental vessel posterior cardinal vein thoracic duct trunk vasculature vascular lymphangioblast
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pkd1a^{hu5855/hu5855} pkd1a^{hu5855/hu5855}; hu5333Tg; y1Tg
Observed In:	heart thoracic duct
Stage:	Day 4

Phenotype Detail

Acknowledgments

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