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ZFIN ID: ZDB-FIG-140708-10
Ramos-Balderas et al., 2013 - The zebrafish mutants for the V-ATPase subunits d, ac45, E, H and c and their variable pigment dilution phenotype. BMC research notes   6:39 Full text @ BMC Res. Notes
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Adult

Fig. 3

RT-PCR of the five V-ATPase subunits in embryos at different developmental stages and in organs from adult fish. (A) RT-PCR from WT embryos at different developmental stages using oligonucleotides that amplify five different V-ATPase subunits. All subunits are consistently expressed throughout all developmental stages, however subunit V1-H is expressed in lower amounts than V0-d1, V0-ac45b, V1-E1b, V1-H and V0-ca. Alpha actin expression was used as an expression control. V0-d1 is abundant in adult males than in females. (B) Semi-quantitative RT-PCR from total RNA obtained from liver, heart, eyes, skin, brain, gut, ovaries and testis from adult zebrafish. The cDNA used was diluted 1:5, 1:100 and 1:1000 and then used for the RT-PCR. V-ATPase subunits V0-ca and V0-ac45b have the higher expression levels, in contrast V0-d1 have the lower expression levels. V1-E1b and V1-H have similar expression; both are enriched in the eyes, brain and ovary.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atp6ap1b WT standard conditions 1-cell unfertilized egg RTPCR
64-cell whole organism RTPCR
50%-epiboly whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult gut RTPCR
Adult heart RTPCR
Adult integument RTPCR
Adult liver RTPCR
Adult ovary RTPCR
Adult testis RTPCR
atp6v0ca WT standard conditions 1-cell unfertilized egg RTPCR
64-cell whole organism RTPCR
50%-epiboly whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult gut RTPCR
Adult heart RTPCR
Adult integument RTPCR
Adult liver RTPCR
Adult ovary RTPCR
Adult testis RTPCR
atp6v0d1 WT standard conditions 1-cell unfertilized egg RTPCR
64-cell whole organism RTPCR
50%-epiboly whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult gut RTPCR
Adult heart RTPCR
Adult integument RTPCR
Adult liver RTPCR
Adult ovary RTPCR
Adult testis RTPCR
atp6v1e1b WT standard conditions 1-cell unfertilized egg RTPCR
64-cell whole organism RTPCR
50%-epiboly whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult gut RTPCR
Adult heart RTPCR
Adult integument RTPCR
Adult liver RTPCR
Adult ovary RTPCR
Adult testis RTPCR
atp6v1h WT standard conditions 1-cell unfertilized egg RTPCR
64-cell whole organism RTPCR
50%-epiboly whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 5 whole organism RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult gut RTPCR
Adult heart RTPCR
Adult integument RTPCR
Adult liver RTPCR
Adult ovary RTPCR
Adult testis RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC research notes for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Res. Notes