FIGURE

Fig. 5

ID

ZDB-FIG-140416-42

Publication

Viringipurampeer et al., 2014 - Rip3 knockdown rescues photoreceptor cell death in blind pde6c zebrafish

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Fig. 5

Flow cytometry sorting of fluorescent cone photoreceptors for gene expression analysis. (a and b) FACS plots for transgenic Tg(TαCP:EGFP) embryos that are wild type at the pde6c^+/+ locus showing the side scatter (SSC) and EGFP profiles. (c and d) FACS plots for mutant Tg(TαCP:EGFP)-pde6c^w59/ cone photoreceptors. (e) Quantification of real-time RT-PCR expression data for rip1 and rip3 from EGFP-positive cells at 4 and 7d.p.f. in photoreceptors from wild-type (wt) and mutant (mt) embryos. Data presented as mean±S.E.M. *P<0.05, n=9. (f) Relative RT-PCR analysis for rod photoreceptor genes (pde6a, pde6c, rho), cone pde6h gamma subunit and β-actin internal control from whole adult retinal RNA (lanes 1 and 6); FACS-sorted mutant cone RNA (lanes 2 and 4); FACS-sorted wild-type cone RNA (lanes 3 and 5). RT, reverse transcriptase

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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