FIGURE

Fig. 2

ID

ZDB-FIG-140403-2

Publication

Cox et al., 2014 - S-Nitrosothiol Signaling Regulates Liver Development and Improves Outcome following Toxic Liver Injury

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Fig. 2

Constitutive NOS Activity Is Required for Optimal Liver Development

(A) Effect of morpholino-mediated knockdown of nos1 and nos2 gene expression on liver formation as assessed by in situ hybridization for lfabp at 72 hpf. Translation blocking ATG morpholinos against nos 1 and 2 were injected at a concentration of 50 μM. Representative photomicrographs were taken at 10× magnification.

(B) Effect of nos knockdown on liver size in Tg(lfabp:GFP) embryos. Representative fluorescent photomicrographs were taken at 10× magnification.

(C) Effect of nos knockdown on hepatic progenitor cells as assessed by in situ hybridization for foxA3 at 48 hpf. Representative photomicrographs were taken at 10× magnification.

(D) Phenotype analysis of liver size (lfabp) in morphants at 72 hpf (n > 50 embryos per treatment).

(E) Effect of nos knockdown on the percentage of hepatocytes specified during liver formation. Tg(lfabp:GFP) morphants were dissociated, and the percentage of GFP positive hepatocytes was analyzed by FACS.

n = 8; ANOVA, p = 0.033 in comparison to control. Results in (E) show mean ± SEM.

Expression Data

Genes:	EGFP fabp10a foxa3
Fish:	WT WT + MO1-nos1 WT + MO1-nos2a mitfa^w2/w2; mpv17^a9/a9; as3Tg mitfa^w2/w2; mpv17^a9/a9; as3Tg + MO1-nos1 mitfa^w2/w2; mpv17^a9/a9; as3Tg + MO1-nos2a
Knockdown Reagents:	MO1-nos1 MO1-nos2a
Anatomical Term:	liver
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-nos1 mitfa^w2/w2; mpv17^a9/a9; as3Tg + MO1-nos1
Knockdown Reagent:	MO1-nos1
Observed In:	hepatocyte liver liver development
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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