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Fig. 2
Constitutive NOS Activity Is Required for Optimal Liver Development
(A) Effect of morpholino-mediated knockdown of nos1 and nos2 gene expression on liver formation as assessed by in situ hybridization for lfabp at 72 hpf. Translation blocking ATG morpholinos against nos 1 and 2 were injected at a concentration of 50 μM. Representative photomicrographs were taken at 10× magnification.
(B) Effect of nos knockdown on liver size in Tg(lfabp:GFP) embryos. Representative fluorescent photomicrographs were taken at 10× magnification.
(C) Effect of nos knockdown on hepatic progenitor cells as assessed by in situ hybridization for foxA3 at 48 hpf. Representative photomicrographs were taken at 10× magnification.
(D) Phenotype analysis of liver size (lfabp) in morphants at 72 hpf (n > 50 embryos per treatment).
(E) Effect of nos knockdown on the percentage of hepatocytes specified during liver formation. Tg(lfabp:GFP) morphants were dissociated, and the percentage of GFP positive hepatocytes was analyzed by FACS.
n = 8; ANOVA, p = 0.033 in comparison to control. Results in (E) show mean ± SEM.