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ZFIN ID: ZDB-FIG-140326-52
Nissen et al., 2003 - Zebrafish foxi one modulates cellular responses to Fgf signaling required for the integrity of ear and jaw patterning. Development (Cambridge, England)   130(11):2543-2554 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-15

Fig. 9 The foo mutation affects pouch formation. Right panels, foo/foo homozygote embryos; left panels, same-clutch wild-type siblings. (A) zn-8 antibody staining of a wild-type embryo at 34 hpf. Pharyngeal pouches (pp1-pp4) are indicated by arrowheads as in Fig. 8. (B) zn-8 antibody staining of a 34 hpf foo/foo embryo. The posterior pouches pp2-4 are substantially reduced, whereas pp1 is only mildly affected. (C) nkx2.3 expression by in situ hybridization of a 30 hpf wild-type embryo. (D) nkx2.3 expression in a 30 hpf foo/foo embryo. Most of the nkx2.3 expression is absent or disorganized. (E) pax8 expression in a 21-somite wild-type embryo with pp2 indicated as in A. (F) pax8 expression in a 21-somite foo/foo embryo. Expression of pax8 in pp2 is absent and the otic vesicle is substantially reduced.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nkx2.3 WT standard conditions Prim-15 pharyngeal pouch ISH
Prim-15 pharyngeal pouch 1 ISH
Prim-15 pharyngeal pouch 2 ISH
Prim-15 pharyngeal pouch 3 ISH
Prim-15 pharyngeal pouch 4 ISH
foxi1hi3747Tg standard conditions Prim-15 pharyngeal pouch ISH
pax8 WT standard conditions 20-25 somites otic vesicle ISH
20-25 somites pharyngeal pouch 2 ISH
foxi1hi3747Tg standard conditions 20-25 somites Not Detected otic vesicle ISH
20-25 somites Not Detected pharyngeal pouch 2 ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-8 IHC WT standard conditions Prim-15 pharyngeal pouch 1
IHC Prim-15 pharyngeal pouch 2
IHC Prim-15 pharyngeal pouch 3
IHC Prim-15 pharyngeal pouch 4
IHC foxi1hi3747Tg standard conditions Prim-15 pharyngeal pouch 1
IHC Prim-15 pharyngeal pouch 2
IHC Prim-15 pharyngeal pouch 3
IHC Prim-15 pharyngeal pouch 4
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development