FIGURE

Fig. S7

ID

ZDB-FIG-140325-24

Publication

Wang et al., 2014 - Notch3 establishes brain vascular integrity by regulating pericyte number

Other Figures

All Figure Page

Back to All Figure Page

Fig. S7

Inhibition of Pdgfr-β activity produces a pericyte deficit and brain hemorrahge. (A) pdgfrb+ brain pericytes in DMSO treated control larvae. (B,C) Larvae treated with 25 μM AG1295 from 32-72 hpf, and from 48-72 hpf, respectively, showed a reduction in pdgfrb+ brain pericyte number. Arrowheads, pdgfrb+ brain pericytes. Scale bar, 200 μm. (A-C) All images show confocal projections of whole brains, viewed from lateral. (D-I) Brain hemorrhage in AG1295 treated zebrafish larvae. (D) DMSO treated control at 3 dpf. (F-I) 25μm AG1295 treated larva at 3 dpf. (E,F,H) Lateral views. (G,I) Dorsal views of (F,H), respectively. (J) notch3^fh332 homozygous mutants showed a more penetrant hemorrhage phenotype when treated with 10 μM AG1295 (n=5, *P<0.05, by paired t-test). Arrowheads, blood pooling at the brain ventricle; open arrowhead, blood pooling behind the eye.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development