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ZFIN ID: ZDB-FIG-140319-74
Schmid et al., 2000 - Equivalent genetic roles for bmp7/snailhouse and bmp2b/swirl in dorsoventral pattern formation. Development (Cambridge, England)   127(5):957-967
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 75%-epiboly

Fig. 5 Expression of bmp2, 4 and 7 in dorsalized mutant embryos at 65-75% epiboly. Expression in wild-type embryos of bmp2b (A), bmp4 (B), and bmp7 (C). Expression of bmp2b, bmp4 and bmp7 in mutant embryos of snhty68a (D-F), sbndtc24 (G-I), swrta72/bmp 2b (JL) and snhsb1aub (M-O), respectively. In snhty68a mutants bmp2b (D) and bmp7 (F) expression are reduced in amount and to a more ventral domain. bmp4 expression is absent in snhty68a(E), sbn (H), swr (K) and snhsb1aub (N) on the ventral side, whereas the dorsal expression domain is unaffected. bmp2b expression is absent in the hypoblast ventrally, but is still present in the YSL and the dorsal margin in sbn/smad5 (G), swr/bmp2b (J) and snhsb1aub (M) mutant embryos. bmp7 expression is reduced in sbn/smad5 (I) and absent in swr/bmp2b mutant embryos, except for expression in the YSL and prechordal plate (L). In snhsb1aub mutants there is no detectable bmp7 expression, including the YSL and prechordal plate expression domains (O).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp2b WT standard conditions 75%-epiboly ectoderm ISH
75%-epiboly margin ISH
75%-epiboly yolk syncytial layer ISH
bmp2bta72a/ta72a standard conditions 75%-epiboly ectoderm ISH
75%-epiboly margin ISH
75%-epiboly yolk syncytial layer ISH
bmp7asb1aub/sb1aub standard conditions 75%-epiboly ectoderm ISH
75%-epiboly margin ISH
75%-epiboly yolk syncytial layer ISH
bmp7aty68a/ty68a standard conditions 75%-epiboly ectoderm ISH
75%-epiboly margin ISH
75%-epiboly yolk syncytial layer ISH
smad5dtc24/dtc24 standard conditions 75%-epiboly ectoderm ISH
75%-epiboly margin ISH
75%-epiboly yolk syncytial layer ISH
bmp4 WT standard conditions 75%-epiboly anterior axial hypoblast ISH
bmp2bta72a/ta72a standard conditions 75%-epiboly anterior axial hypoblast ISH
bmp7asb1aub/sb1aub standard conditions 75%-epiboly anterior axial hypoblast ISH
bmp7aty68a/ty68a standard conditions 75%-epiboly anterior axial hypoblast ISH
smad5dtc24/dtc24 standard conditions 75%-epiboly anterior axial hypoblast ISH
bmp7a WT standard conditions 75%-epiboly ectoderm ISH
75%-epiboly endoderm ISH
75%-epiboly yolk syncytial layer ISH
bmp2bta72a/ta72a standard conditions 75%-epiboly ectoderm ISH
75%-epiboly endoderm ISH
75%-epiboly yolk syncytial layer ISH
bmp7asb1aub/sb1aub standard conditions 75%-epiboly ectoderm ISH
75%-epiboly endoderm ISH
75%-epiboly yolk syncytial layer ISH
bmp7aty68a/ty68a standard conditions 75%-epiboly ectoderm ISH
75%-epiboly endoderm ISH
75%-epiboly yolk syncytial layer ISH
smad5dtc24/dtc24 standard conditions 75%-epiboly ectoderm ISH
75%-epiboly endoderm ISH
75%-epiboly yolk syncytial layer ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.