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ZFIN ID: ZDB-FIG-140319-30
Neumann et al., 1999 - Transient establishment of anteroposterior polarity in the zebrafish pectoral fin bud in the absence of sonic hedgehog activity. Development (Cambridge, England)   126(21):4817-4826
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-15 to Prim-25

Fig. 1 Expression of ptc1 and hoxd genes in wildtype and syu pectoral fin buds. Whole-mount in situ hypbridizations. Anterior is to the the left and distal is to the top. (A-D,G,H,K,L,O,P) 32 hour pectoral fin buds. (E,F,I,J,M,N,Q,R) 38 hour pectoral fin buds. (A,C,E,G,I,K,M,O,Q) Wild-type fin buds; (B,D,F,H,J,L,N,P,R) syu fin buds. (A,B) ptc1 RNA; (C-F) hoxd-10 RNA; (G-J) hoxd-11 RNA; (K-N) hoxd- 12 RNA; (O-R) hoxd-13 RNA. At 32 hours, ptc1 and hoxd-13 are not expressed in syu buds, while hoxd-10 appears normal and hoxd-11 and hoxd-12 show a partial reduction. At 38 hours only expression of hoxd-10 remains in syu mutant buds.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hoxd10a WT standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
shhaDf(Chr07)t4 standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
hoxd11a WT standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
shhaDf(Chr07)t4 standard conditions Prim-15 Not Detected pectoral fin bud ISH
Prim-25 Not Detected pectoral fin bud ISH
hoxd12a WT standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
shhaDf(Chr07)t4 standard conditions Prim-15 Not Detected pectoral fin bud ISH
Prim-25 Not Detected pectoral fin bud ISH
hoxd13a WT standard conditions Prim-15 pectoral fin bud ISH
Prim-25 pectoral fin bud ISH
shhaDf(Chr07)t4 standard conditions Prim-15 Not Detected pectoral fin bud ISH
Prim-25 Not Detected pectoral fin bud ISH
ptch2 WT standard conditions Prim-15 pectoral fin bud ISH
shhaDf(Chr07)t4 standard conditions Prim-15 Not Detected pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.