FIGURE

Fig. 6

ID

ZDB-FIG-140226-14

Publication

Essers et al., 2014 - A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis

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Fig. 6

Loss of p53 partially rescues mutant phenotypes. (A) Bright field microscopy of p53 MO injections at 2 dpf. UIC=uninjected control embryos. Arrows depict inflation of the hindbrain ventricle, arrowheads depict enlargement of the forebrain. The ragged skin phenotype in gnl3l mutants and the rescue are depicted with the black bars. (B) Representative profiles of mutant embryos injected with the p53 MO. (C) Magnified representation of polysome profiles showing an increase in 60S peak size in p53 MO compared to missense MO injected embryos. (D) Quantification of the ratio of 60S to 40S peak size in missense vs. p53 MO injected embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gnl2^bw41c/bw41c gnl3^{hu3259/hu3259} gnl3l^{hi1437Tg/hi1437Tg} gnl2^bw41c/bw41c + MO4-tp53 gnl3^{hu3259/hu3259} + MO4-tp53 gnl3l^{hi1437Tg/hi1437Tg} + MO4-tp53
Knockdown Reagent:	MO4-tp53
Observed In:	forebrain ventricle fourth ventricle integument ribosome biogenesis whole organism cytosolic ribosome
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 385(2), Essers, P.B., Pereboom, T.C., Goos, Y.J., Paridaen, J.T., and Macinnes, A.W., A comparative study of nucleostemin family members in zebrafish reveals specific roles in ribosome biogenesis, 304-15, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.