FIGURE

Fig. 2

ID
ZDB-FIG-140204-28
Publication
Kapp et al., 2013 - The integrator complex subunit 6 (ints6) confines the dorsal organizer in vertebrate embryogenesis
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Reduced bmp expression but intact BMP signal transduction in p18ahub mutants.

(A–H) In situ hybridization on early gastrula stage embryos or equivalent, except (C) and (D) are mid gastrula stage or equivalent. Embryos were stage-matched. Animal pole views, dorsal to the right for all. Expression of bmp ligand gene in WT embryos (A, n = 32, C, n = 43, and E, n = 21) and p18ahub embryos (B, n = 30, D, n = 18, and F, n = 16). Expression of the BMP antagonist chordin (chd) was confined to the dorsal side of WT embryos (G, n = 21) but was expanded around the entire margin of p18ahub embryos (H, n = 20) by early gastrulation. (I–N) Pie charts indicate fractions of embryos at 24 hpf that displayed the indicated phenotypes (categories described in the text). Number of embryos is shown at the center of each chart. p18ahub embryos (I, O, uninjected) that were injected with 20 pg bmp2b mRNA were rescued to WT or ventralized (K, P, +bmp2b mRNA) similarly to WT embryos (J, +bmp2b mRNA). p18ahub mutant embryos (L, uninjected) depleted of Chordin, Noggin1, and Follistatin-like 2b proteins by MO injection were similarly ventralized (N, +MOcnf) to WT embryos (M, +MOcnf).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.