FIGURE

Fig. 1

ID

ZDB-FIG-140117-17

Publication

Nagashima et al., 2013 - A self-renewing division of zebrafish Muller glial cells generates neuronal progenitors that require N-cadherin to regenerate retinal neurons

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Fig. 1

Alcama is a novel marker of multipotent retinal stem cells in adult zebrafish. (A) PCNA⁺ progenitors (red) in the CMZ and nGFP⁺ immature Müller glia (arrowheads) in the INL express Rx1 (magenta). (B) GFP⁺ Müller glia express rlbp1a transcripts (magenta). (C) Retinal progenitors and immature Müller glia express BLBP (white/magenta). (D) Alcama (white/magenta) in PCNA⁺ (green) cells in the CMZ (boxed region shown in the overlay in the middle panel). Differentiating RGCs are indicated by asterisks. (E) Alcama⁺ cells (white/magenta) in the INL are nGFP⁺ immature Müller glia (boxed regions are shown at higher magnification in the insets at bottom right). (F) Alcama (white/magenta) colocalizes with a GFP⁺ Müller glial process (boxed regions shown at higher magnification in the insets at the upper left). Scale bars: 10 μm.

Expression Data

Genes:	alcama EGFP GFP rlbp1a
Antibodies:	Ab1-rx1 Ab3-fabp7 Ab4-pcna zn-5
Fish:	WT mi2002Tg mi2004Tg
Anatomical Terms:	ciliary marginal zone Muller cell Muller cell cytoplasm retinal ganglion cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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