FIGURE

Fig. 7

ID

ZDB-FIG-140117-23

Publication

Nagashima et al., 2013 - A self-renewing division of zebrafish Muller glial cells generates neuronal progenitors that require N-cadherin to regenerate retinal neurons

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Fig. 7

Reduction of N-cadherin function interferes with formation of neurogenic clusters after light lesions. (A) N-cadherin (white/magenta) at OLM (between arrowheads) in unlesioned (ctrl) retina. (B) At 3 dpl, N-cadherin (white/magenta) in Müller glia and progenitors (green). (C) RT-PCR products of cdh2 digested with BsmBI. (D,E) N-cadherin (white/magenta) in the ONL at 3 dpl in cdh2⁺^/^m117; mi2002 (D) and cdh2⁺^/^m117; mi2004 (E). (F) Neurogenic clusters (PCNA⁺; green) in sib retinas at 3 dpl. (G) PCNA⁺ (green)/BLBP⁺ (red)/Rx1⁺ (white/magenta) progenitors in cdh2⁺^/^m117 hets at 3 dpl. (H) Counts of PCNA⁺ progenitors in the INL and ONL at 3 dpl. ^*P<0.005, ^**P<0.0005. (I) Nuclei of nGFP⁺/Alcama⁺ (white/magenta) Müller glia in the INL and nGFP⁺/Alcama^- progenitors in the ONL at 3 dpl in cdh2⁺^/^m117; mi2004. (J) pH3⁺ (magenta) nuclei at 3 and 5 dpl in sibs. (K) pH3⁺ (magenta) nuclei at 3 and 5 dpl in cdh2⁺^/^m117 hets. (L) Fraction of pH3⁺ cells in INL and ONL after lesion. ^*P<0.0001. Box plots: median, 25th and 75th percentiles; whiskers show maximum and minimum data points. Scale bars: 20 μm.

Expression Data

Genes:	cdh2 EGFP GFP
Antibodies:	Ab1-cdh2 Ab1-elavl Ab1-rx1
Fish:	WT cdh2^m117/+ mi2002Tg mi2004Tg cdh2^m117/+; mi2002Tg cdh2^m117/+; mi2004Tg (all 6)
Condition:	chemical ablation: retinal neural layer
Anatomical Terms:	amacrine cell Muller cell retinal cone cell retinal ganglion cell retinal inner nuclear layer retinal inner nuclear layer neuron (all 6)
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdh2^m117/+ cdh2^m117/+; mi2002Tg cdh2^m117/+; mi2004Tg
Condition:	chemical ablation: retinal neural layer
Observed In:	retina sensory epithelium regeneration retinal ganglion cell retinal ganglion cell layer
Stage:	Adult

Phenotype Detail

Acknowledgments

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