FIGURE

Fig. 5

ID

ZDB-FIG-140114-32

Publication

Wilkins et al., 2013 - p53-Mediated Biliary Defects Caused by Knockdown of cirh1a, the Zebrafish Homolog of the Gene Responsible for North American Indian Childhood Cirrhosis

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Fig. 5

Cirhin-deficient larvae have defects in biliary and canalicular morphology.

(A-C) Confocal projections of 5 dpf livers immunostained with antibodies against canalicular marker MDR (red) and biliary epitope 2F11 (green). Livers from fish with normal BODIPY-FL C16 processing (A) show elongated canaliculi intersecting with normally arborized biliary networks. In contrast, fish with reduced fluorescent lipid processing (B and C) show truncated, rounded canalicular profiles and more sparse biliary networks. Insets show high-magnification view of one or few cells to highlight canalicular morphology. (D) Semiquantitative scoring of canalicular morphology, with 1+=0-25% elongated, 2+=26-50% elongated, 3+=51-75% elongated, and 4+=76-100% elongated. *, p<0.05 vs Mismatch MO 1 ng.

Expression Data

Antibodies:	Ab-2F11 Ab1-mdr
Fish:	WT WT + MO1-utp4 WT + MO2-utp4
Knockdown Reagents:	MO1-utp4 MO2-utp4
Anatomical Terms:	bile canaliculus bile ductule
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-utp4 WT + MO2-utp4
Knockdown Reagents:	MO1-utp4 MO2-utp4
Observed In:	bile canaliculus bile ductule
Stage:	Day 5

Phenotype Detail

Acknowledgments

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